Open AccessAnti-microbial and anti-inflammatory flavonoid constituents from the leaves of Lawsonia inermis
Author(s) -
Manivannan Rajamanickam,
R Aeganathan,
K. Prabakaran
Publication year - 2015
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2015.4404
Subject(s) - lawsonia inermis , antimicrobial , apigenin , chemistry , traditional medicine , flavonoid , carrageenan , anti inflammatory , escherichia coli , biology , food science , biochemistry , antioxidant , pharmacology , medicine , organic chemistry , gene
A chemical investigation on the leaves of Lawsonia inermis led to the isolation and identification of five known and rare flavonoids, apigenin (1), 5, 7, 4 - trihydroxy-6, 3, 5-trimethoxyflavone (2), pectolinarigenin (3), apigenin-7-O-β-D-glucoside (4), and pectolinarin (5). Their structures were established on the basis of chromatography and spectroscopic evidence and comparisons with literature data. Antimicrobial and carrageenan induced paw edema in male albino rats were determined. Compounds 4 and 5 showed the most powerful antimicrobial effect against Escherichia coli and Aspergillus flavus at the dose of 200 mg/kg. Compound 2 showed pronounced anti-inflammatory effects (3.17±0.15 after 3 h of injection), slightly higher than that of other four compounds.