Open AccessPreliminary phytochemical examination of Homonoia riparia Lour. and its effect on clotting time in vitro
Author(s) -
Vishwas Kabbinale,
Suchitra Narayan Prabhu,
K N Sunil Kumar,
Naveen Chandra,
B Ravishankar,
B Yashovarma
Publication year - 2015
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2015.4308
Subject(s) - phytochemical , terpenoid , traditional medicine , clotting time , glycoside , prothrombin time , chemistry , medicine , botany , biology , platelet , surgery , immunology
Whole plant parts of Homonoia riparia Lour. (Euphorbiaceae) is claimed to be active against various ailments like constipation, emesis, piles, bladder stones, gonorrhoea, syphilis, toothache, angina, malaria, and wounds caused by scorpion and fish bites. It is claimed to have blood clotting property as leaves are used to stop bleeding in fresh cut wounds in folklore practice. The present study was carried out to evaluate the phytochemical composition and effect of its extracts on clotting time. Leaf sample of H. riparia was standardized for authenticity, quality and chemical composition using Pharmacopoeial procedures. Preliminary phytochemical tests were performed using procedures of phytochemical testing. Chloroform and ethanol extracts of air dried leaf at different dose levels were tested on clotting time on blood of healthy volunteers following Lee and White method. Physico-chemical constants and HPTLC fingerprint of the H. riparia leaf was recorded. Preliminary phytochemical tests revealed presence of alkaloids, carbohydrates/glycosides, carboxlic acids, flavonoids, phenols, steroids, saponins, tannins and terpenoids. The ethanolic extract at 100 mg was found to be better than chloroform extract. The activity is found to increase with increase in concentration. Standardized H. riparia investigated in the current study possess anticoagulant activity in contrast to the claim of blood clotting activity claimed in folk medicine.