Open AccessAntioxidant and hepatoprotective effect of Macrotyloma uniflorum seed in antitubercular drug induced liver injury in rats
Author(s) -
Vandana Panda,
K Kundnani,
P. S. Kharat,
S Sudhamani
Publication year - 2015
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2015.4105
Subject(s) - malondialdehyde , antioxidant , chemistry , glutathione reductase , glutathione , lipid peroxidation , glutathione peroxidase , superoxide dismutase , liver injury , pharmacology , biochemistry , catalase , bilirubin , enzyme , biology , endocrinology
Horse gram [Macrotyloma uniflorum Lam. (Verdc.)] is a lesser known bean consumed as a whole seed or sprout in India. Its seeds are rich in flavonoids and phenolic acids. The protective effect of a hydroalcoholic extract of the seed of Macrotyloma uniflorum (MUSE) in anti-tubercular drug (ATD) induced liver injury and the probable mechanism involved in this protection was investigated in rats. The phenolic acids viz., ferulic and p-coumaric acid were isolated and quantified from MUSE. MUSE (250 mg/kg and 500 mg/kg) and the reference drug Liv.52 (500 mg/kg) was administered orally for 30 days to ATD (isoniazid 7.5 mg/kg, rifampicin 10 mg/kg and pyrazinamide 35 mg/kg) treated rats. Levels of marker enzymes (AST, ALT, ALP and γ-GT), albumin, total proteins and bilirubin were estimated in serum. Activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase), reduced glutathione levels and the lipid peroxidation marker malondialdehyde were determined in liver. MUSE elicited significant hepatoprotective and antioxidant activity by attenuating the ATD–elevated levels of the marker enzymes, bilirubin and malondialdehyde and restored the ATD–depleted levels of albumin, total proteins, reduced glutathione and the antioxidant enzymes. The present findings indicate that the hepatoprotective effect of GIE in ATD–induced oxidative damage may be due to its antioxidant activity.