Open AccessAntimicrobial, antioxidant and wound healing properties of Leucas lanata Wall. ex Benth
Author(s) -
Veena Dixit,
Pritt Verma,
Priyanka Agnihotri,
A. K. Paliwal,
Chandana Venkateswara Rao,
Tariq Husain
Publication year - 2015
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2015.4103
Subject(s) - antimicrobial , traditional medicine , wound healing , antioxidant , staphylococcus epidermidis , chemistry , superoxide dismutase , catalase , biology , medicine , staphylococcus aureus , biochemistry , bacteria , genetics , immunology , organic chemistry
Leucas lanata Wall. ex Benth. (Lamiaceae) is an important plant commonly known as Biskapra or Gumma and used to treat many ailments by traditional healers and local peoples. This study was designed to evaluate wound healing potential of Leucas lanata through the excision wound model and functional changes in biochemical indicators of antioxidant parameters. This study also investigated the antimicrobial as well as antioxidant activity of L. lanata. The 50% EtOH extract of L. lanata was found to contain 0.74% of phenolic and 0.21% of flavonoid content. The IC5O value was 122.56 μg/ml and reducing power increased with the increasing concentrations. The antimicrobial activity of the extract was more effective against bacterial strains compared to fungal strains. Remarkable wound healing activity was observed with the 10% (w/w) ointment of L. lanata 50% EtOH extract. In the study of uninfected wounds, epithelization period was reduced from 24.66±0.97 for the control group treated with blank ointment to 12.16±0.36 for the group treated with 10% LLEE ointment. Similarly, in case of infected wounds with Staphylococcus epidermidis, the percentage of wound contraction was significantly enhanced. Both doses of extract significantly increased superoxide dismutase, catalase, reduced glutathione when compared with the control group of infected and uninfected wound. HPLC analysis showed the presence of gallic, protocatechuic, chlorogenic, caffeic and ferulic acids. These compounds have important biological activities and responsible for antimicrobial and wound healing activity. The study provided sufficient evidences that, L. lanata might be indeed potential sources to treat many diseases.