
Evaluation of Salvia haematodes Linn for their antioxidant, anti-microbial, anti-lipoxygenase and anticholinesterase activity
Author(s) -
Rizwan Ali,
Rao Saeed,
Taseer Ahmadv,
Aftab Ullah,
Taseer Ahmad,
Zaira Ather,
Sohail Muhammad,
Usman Ali
Publication year - 2014
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2014.3503
Subject(s) - chemistry , dpph , antioxidant , maceration (sewage) , chromatography , butyrylcholinesterase , acetylcholinesterase , lipoxygenase , enzyme , aché , biochemistry , materials science , composite material
The indigenous medicinal plant Salvia haematodes Linn (SAL) was screened for its pharmacological activities. The crude extract fractions were prepared in methanol, n-hexane, di chloromethane (DCM) and n-butanol by cold maceration process. The antioxidant effect of the extract fractions was observed by 1-1- diphenyl 2-picryl hydrazine (DPPH) radical scavenging activity. The anti-microbial activity was observed by using disc diffusion method. Furthermore, anti-enzymatic activity was studied against LOX (anti-lipoxygenase), acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). The results exhibited that the n-hexane (0.5 mg/mL) and DCM (0.5 mg/mL) fractions of SAL showed 83.3 ± 0.8% and 91.56 ± 0.7 % inhibition of DPPH radical. Whereas, the n-butanol fraction of the crude extract did not show any effect. The methanolic extract (10 mg/disc) of SAL inhibited the growth of E. coli at 12±0.5 mm as compared to ciprofloxacin (17.3 ± 0.5 mm). Moreover, at concentration of 0.5 mg/mL, the n-hexane and DMC extracts of SAL inhibited 68.19±0.39 % and 75.8 ± 1.7% of LOX enzyme respectively, against standard Baicallin (93.7 ± 1.2 % ). The methanol fraction of SAL showed 77.95 ± 0.6 % of AChE inhibition, whereas, Eserine standard inhibited 91.2 ±1.1% of AChE. The methanolic fraction of SAL showed 51.9±0.4 % inhibition of BChE enzyme against standard Eserine (82.8±1.0%). However, the DCM and n-hexane fractions did not elucidate any activity. Therefore, it is concluded that SAL have different pharmacology activities that may be due to presence of certain pharmacologically active compound.