Open AccessCOX and LOX inhibitory potential of Abroma augusta and Desmodium gangeticum
Author(s) -
Raj Kishor Bisht,
Sayak Bhattacharya,
Yusuf Ali Jaliwala
Publication year - 2014
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2014.3303
Subject(s) - ic50 , arachidonate 5 lipoxygenase , chemistry , cyclooxygenase , in vivo , inhibitory postsynaptic potential , pharmacology , carrageenan , lipoxygenase , biochemistry , enzyme , in vitro , biology , arachidonic acid , microbiology and biotechnology , endocrinology
The present study was aimed to evaluate the cyclooxygenase (COX) and lipoxygenase (LOX) inhibitory activity of Abroma augusta (AA) and Desmodium gangeticum (DG). Initially In-vivo anti-inflammatory activity of aqueous extract (AqE) of aerial parts of DG (100 mg/ml) and petroleum ether extract (PEE) of roots of AA (250 mg/ml) was assessed in carrageenan induced paw oedema model in albino wistar rats. In addition, each plant extract was evaluated for COX1, COX-2 and LOX inhibitory activity to determine the possible mechanism of their antiinflammatory activity. The results of the study demonstrated that each plant extract significantly (p<0.0001) reduces the paw volume compared with standard drug Ibuprofen (100 mg/kg b.w.). The percentage inhibitory activity of AqE of DG against COX-2 (IC50=39. 5 µg/ml) were generally higher to that of COX-1 (IC50=49. 5 µg/ml), however, at the same concentrations PEE of AA demonstrated inhibitory activity against COX-1 (IC50=36. 5 µg/ml) to a greater extent than COX-2 (IC50=59 µg/ml). Moderate inhibition of LOX activity was demonstrated by DG (IC50=57.0 µg/ml). AA exhibited weak inhibitory action on LOX activity at the same concentrations (IC50=75.5 µg/ml). The results of the study concluded that anti-inflammatory activity of plant extracts could be due to inhibition of COX and LOX enzymes and thus supports the traditional use of the above mentioned plants in inflammatory disorders.