Open AccessDocking study of the Rohitukine for the prevention of peptic ulcer- A New Target
Author(s) -
Sunil Kumar Mishra,
Vaibhav Mishra,
Gautam Palit,
Anil Kumar Pandey,
Akanksha Srivastava,
Snober S. Mir,
Abbas Ali Mahdi,
Vijai Lakshmi,
Ckm Tripathi,
Snober S. Mir
Publication year - 2014
Publication title -
the journal of phytopharmacology
Language(s) - English
Resource type - Journals
ISSN - 2320-480X
DOI - 10.31254/phyto.2014.3102
Subject(s) - histamine , secretion , mechanism of action , gastric acid , docking (animal) , context (archaeology) , pharmacology , chemistry , peptic , peptic ulcer , medicine , biochemistry , in vitro , biology , paleontology , nursing
Context: In our previous study we have suggested that Rohitukine attenuates gastric mucosal injury; however its exact mechanism has not yet been established. Objective: The aim of present study was to evaluate the gastro protective mechanism of Rohitukine. Materials and methods: Sprague dawley rats and guinea pigs weighing 180–200 g were used. Dudenol ulcer was observed through Histamine induced gastric ulcer in guinea pigs. Result and Discussion: The present study was considered to evaluate the anti-ulcerogenic properties of an alkaloid chromane, Rohitukine from Dysoxylum binectariferum. Moreover, we studied the role of Rohitukine on the cytosolic concentration of cAMP and Histamine level in parietal cell-enriched cell suspension in order to ascertain its mechanism of action. In addition, Rohitukine failed to show protection in histamine induced gastric ulcer. Conclusion: Our docking study revealed that Rohitukine moderately bind with CCK2 receptor with binding energy as compare with standard drug benzotript. Furthermore, anti-secretory mechanism of Rohitukine mediated apparently through bind with CCK2 and inhibited the acid secretion, is novel to our finding.