Open AccessIONIC HOMEOSTASIS IN WHEAT PLANTS INOCULATED BY HORMONE-PRODUCING BACTERIA UNDER SALINITY
Author(s) -
Elena Martynenko,
Т. Н. Архипова,
А. А. Белимов,
G. R. Kudoyarova
Publication year - 2020
Publication title -
èkobioteh
Language(s) - English
Resource type - Journals
ISSN - 2618-964X
DOI - 10.31163/2618-964x-2020-3-4-727-733
Subject(s) - salinity , shoot , rhizosphere , sodium , potassium , bacillus subtilis , cytokinin , chemistry , inoculation , bacteria , ionic bonding , horticulture , botany , auxin , biology , ion , biochemistry , ecology , genetics , organic chemistry , gene
Сytokinins content, concentration of sodium and potassium ions and K+/Na+ratio in wheat plants (Triticum durum Desf., Bashkirskaya 27) were evaluated under salinity during treatment with hormone-producing bacteria Pseudomonas mandelii IB-Ki14 (auxin-producer) or Bacillus subtilis IB-22 (cytokinin-producer). An increased level of cytokinins was observed in the roots on 6th day and then in the shoots (on 11th day) both in the absence of salt and under 100 mM NaCl in the presence of B. subtilis IB-22. The introduction of P. mandelii IB-Ki14 into the rhizosphere did not lead to the accumulation of cytokinins under salinity. The presence of NaCl in the soil led to the expected increase in the concentration of sodium ions both in the roots and in the shoots, and inoculation did not significantly change their values. Salinity decreased the content of potassium ions in the roots by 25% in plants uninoculated with bacteria and in plants treated with P. mandelii IB-Ki14, as a result of which the ratio of K+/Na+ ions decreased by 4 times compared with the control, indicating a disruption of the ionic homeostasis. In plants inoculated with B. subtilis IB-22, no decrease in the concentration of potassium ions was found as compared to the control, and the ratio of K+/Na+ ions in the roots under salinity decreased to a lesser extent than in other variants of the experiment. This indicates the ability of cytokinin-producing bacteria to stabilize ionic balance and reduce the consequences of the negative effects of salinity on photosynthesis, protein synthesis, and growth. Our data indicate that this property of bacteria is associated with their ability to synthesize cytokinins and increase cytokinin content in plants under salinity.