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E‐Selectin Is Upregulated in Proliferating Endothelial Cells In Vitro
Author(s) -
Bischoff Joyce,
Brasel Christopher,
Kräling Birgit,
Vranovska Katerina
Publication year - 1997
Publication title -
microcirculation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.793
H-Index - 83
eISSN - 1549-8719
pISSN - 1073-9688
DOI - 10.3109/10739689709146791
Subject(s) - endothelial stem cell , e selectin , propidium iodide , cell cycle , cell sorting , biology , cell adhesion , angiogenesis , microbiology and biotechnology , endothelium , cell , chemistry , flow cytometry , in vitro , apoptosis , biochemistry , cancer research , endocrinology , programmed cell death
Objective: E‐selectin is an endothelial cell‐specific membrane glycoprotein that participates in leukocyte adhesion and has also been suggested to function in angiogenesis. To gain further insights into E‐selectin, we analyzed E‐selectin polypeptide in proliferating versus quiescent bovine capillary endothelial cells and its expression as a function of the cell cycle. Methods: E‐selectin polypeptide was analyzed by immunoadsorption from 35 Scysteine—labeled endothelial cells, by enzyme‐linked immunosorbent assay, and by fluorescence‐activated cell sorting. The distribution of endothelial cells in G 0 /G 1 , S, and G 2 /M phases of the cell cycle was determined using propidium iodide staining of DNA. Results: E‐selectin was upregulated in subconfluent proliferating bovine capillars endothelial cells compared to confluent quiescent cultures. The upregulation was independent of activation in that E‐selectin was further increased by treatment with tumor necrosis factor α or lipopolysaccharide. In contrast to E‐selectin, P‐selectin and platelet‐endothelial cell adhesion molecule‐1 did not appear to be regulated by the growth state of the endothelial cells. The distribution of E‐selectin‐positive cells in G 0 G 1 , S, and G 2 /M phases of the cell cycle differed from E‐selectin‐negative cells in that more of the E‐selectin‐positive cells were in G 2 and M. Conclusions: Increased E‐selectin expression under noninflammatory conditions is correlated with cellular proliferation and G 2 /M phases of the cell cycle. The expression of E‐selectin in proliferating endothelial cells in vitro is consistent with the presence of E‐selectin in proliferating endothelial cells in vivo (Kräling et al. [18]).