Open AccessUrinary trypsin inhibitor may have a protective effect on endothelial cells in preeclampsia
Author(s) -
Maradny Emad El,
Kanayama Naohiro,
Halim Abdul,
Maehara Kayoko,
Terao Toshihiko
Publication year - 1994
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.3109/00016349409072499
Subject(s) - preeclampsia , amniotic fluid , medicine , endocrinology , umbilical vein , urine , urinary system , neutrophil elastase , trypsin inhibitor , trypsin , fetus , pregnancy , andrology , enzyme , biology , biochemistry , inflammation , in vitro , genetics
Urinary trypsin inhibitor is a glycoprotein which has an inhibitory effect on many enzymes, especially neutrophil elastase. The concentration of urinary trypsin inhibitor in serum (maternal and fetal), urine and amniotic fluid was measured in 20 cases of preeclampsia and compared with levels in normal pregnancy. Urinary trypsin inhibitor levels were significantly increased in the maternal and fetal serum of preeclamptic patients compared to normal pregnancy ( p < 0.0001), as well as in urine and amniotic fluid of the same patients. Relative fluorescence release of a calcium chelating agent (fura‐2) from human umbilical vein endothelial cell cultures was significantly increased by preeclamptic serum compared to serum of normal pregnant women ( p < 0.03). After incubation of urinary trypsin inhibitor with the cultures, significant decrease of fura‐2 release was observed ( p < 0.03). Urinary trypsin inhibitor has an effect on suppression of activated neutrophils, elastase production and may have a protective effect on endothelial cells.