Open Access‘Hit and run’ oncogenesis by human papillomavirus type 18 DNA
Author(s) -
Iwasaka T.,
Hayashi Y.,
Yokoyama M.,
Hara K.,
Matsuo N.,
Sugimori H.
Publication year - 1992
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.3109/00016349209009922
Subject(s) - southern blot , carcinogenesis , transfection , cell culture , virology , biology , microbiology and biotechnology , virus , hamster , dna , transformation (genetics) , polymerase chain reaction , cell , gene , genetics
Transfection of an immortalized cell line (AE), derived from Syrian hamster embryo cells, with human papillomavirus type 18 (HPV 18) DNA induced morphological transformation and these transformed cells were tumorigenic in nude mice. Southern blot analysis revealed that the transfected viral DNA was retained in all the cell lines tested, however, all these transformed cells contained only less than one copy per cell of viral genome. Eleven cloned cell lines were established from a tumor cell line obtained after explantation of a tumor into a nude mouse. Two lines revealed no viral sequences by both Southern blot hybridization and polymerase chain reaction, whereas the nine others contained the remaining viral sequences. These results are highly suggestive of a ‘hit and run’ oncogenesis by this virus.