Open AccessEstablishment and Characterization of a Subline Predisposed to Pulmonary Metastasis from a Human Gestational Choriocarcinoma Cell Line in Nude Mice
Author(s) -
Kanazawa Koji,
Adachi Shigemi,
Yoshiya Norio,
Honma Shigeru,
Takahashi Hiroaki,
Takeuchi Shoshichi
Publication year - 1989
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.3109/00016348909021015
Subject(s) - medicine , choriocarcinoma , metastasis , chorioepithelioma , gestation , gynecology , cell culture , pregnancy , oncology , obstetrics , cancer research , pathology , cancer , biology , genetics
A subline predisposed to pulmonary metastasis was successfully derived by repeating in vivo selection of GCH‐1, a human gestational choriocarcinoma cell line, in nude mice. While the parent line GCH‐1, transplanted subcutaneously to nude mice, induced pulmonary metastasis in a few of the host animals, the newly established subline GCH‐l(m) successfully metastasized to the lungs in 100% of them. Compared with GCH‐1, GCH‐l(m) exhibited a higher degree of cell atypia, a lower capacity for cell growth and markedly higher productivity for human chorionic gonadotropin (hCG). Another characteristic of this subline was its enhanced growth after the addition of a pulmonary extract obtained from nude mice. The relationship of these findings to the mechanisms of metastasis of this cancer was discussed.