Open AccessThe Effect of Luteinizing Hormone Releasing Hormone (Lhrh) on Steroidogenesis by the Human Luteal Tissue
Author(s) -
Ikoma Hisao,
Yamoto Mareo,
Nakano Ryosuke
Publication year - 1987
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.3109/00016348709004145
Subject(s) - luteal phase , endocrinology , luteinizing hormone , human chorionic gonadotropin , medicine , corpus luteum , basal (medicine) , gonadotropin , hormone , basal body temperature , biology , menstrual cycle , insulin
The effect of luteinizing hormone releasing hormone (LHRH) on progesterone and estradiol production was studied in human luteal tissue. Tissue slices were incubated in Ham's F‐10 medium under an atmosphere of 95% O 2 ; 5% CO 2 at 37 °C for 4 h. Slices were treated with or without human chorionic gonadotropin (HCG) in the presence or absence of LHRH. HCG stimulated progesterone and estradiol production by human luteal tissue in a time‐and dose‐related manner. LHRH stimulated the basal progesterone production in five (one early luteal and four mid‐luteal) of 17 corpora lutea, and stimulated the basal estradiol production in three (all mid‐luteal) of 16 corpora lutea. On the other hand, LHRH inhibited HCG–stimulated progesterone production in four (two early heal and two mid–luteal) of 22 corpora lutea. and inhibited HCG‐stimulated estradiol production in four (one early luteal and three mid‐luteal) of 18 corpora lutea. In the acute short‐term incubations of human luteal tissue, therefore, LHRH had little effect on basal and HCG‐stimulated steroidogenesis. However, in a few corpora lutea taken in the early or mid‐luteal phase, LHRH 5timulated basal steroidogenesis and inhibited HCG‐stimulated steroidogenesis.