Open AccessIn Vitro Perfusion Studies on Human Umbilical Arteries: I. Vasoactive Effects of Serotonin, PGF 2α and PGE 2
Author(s) -
Bjøro Kristian,
StrayPedersen Sverre
Publication year - 1986
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.3109/00016348609157359
Subject(s) - methysergide , serotonin , vasoconstriction , perfusion , umbilical artery , medicine , vasoactive , endocrinology , umbilical cord , anesthesia , anatomy , biology , fetus , genetics , pregnancy , receptor
A method was developed for the assessment of in vitro perfusion of the umbilical cord arteries. a perfusion pressure of 60–80 mmHg gave flow rates ranging from 30 to 40 ml/min per artery. Serotonin, PGF 2α and PGE 2 were added to the perfusate and tested for vasoactivity. All substances induced a dose‐dependent vasoconstriction. Serotonin proved to be the most potent vasoconstrictor. the minimum dose required to induce visible pressure responses was 10 −9 –10 −8 M of serotonin and 10 −7 M of PGF 2α and PGE 2 . When adding methysergide to the perfusate, the serotonin response was abolished, while the effects of the prostanoids remained unaltered.