Open AccessScreening of endometrial carcinoma by lactate dehydrogenase isoenzyme analysis of uterine fluid
Author(s) -
Niklasson O.,
Skude G.,
Johansson R.,
Stormby N.
Publication year - 1981
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.3109/00016348109154100
Subject(s) - medicine , lactate dehydrogenase , isozyme , cytology , carcinoma , population , pathology , endometrium , gynecology , enzyme , biology , biochemistry , environmental health
Determination of total activity for lactate dehydrogenase (LD) as well as electrophoretic separation and quantitative determination of the individual LD‐isoenzymes was performed in 431 samples of uterine fluid obtained by the Gravlee Jet Wash technique or a described simple device for endouterine aspiration. The isoenzyme pattern for each menstrual phase was characteristic. The study population included 18 patients with endouterine neoplasia. All these malignancies were characterized by increased total LD‐activity and/or abnormal distribution of the individual LD‐isoenzymes compared to proliferative phase and would consequently have been discriminated at postmenstrual or postmenopausal examination. The LD‐enzyme technique proved to be more accurate than cytology with approximately the same number of false positives.