Open AccessDisc‐Electrophoretic Protein Pattern of Cervical Mucus in Cases of Humoral Sensitization Against Spermatozoa
Author(s) -
Mettler L.,
Gradl T.,
Mäder Ch.
Publication year - 1976
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.3109/00016347609156780
Subject(s) - ouchterlony double immunodiffusion , cervical mucus , sperm , mucus , immunoelectrophoresis , andrology , polyacrylamide gel electrophoresis , albumin , ovalbumin , medicine , electrophoresis , semen , blood proteins , antibody , immunology , antigen , biology , ovulation , endocrinology , biochemistry , antiserum , ecology , hormone , enzyme
. Midcycle cervical mucus samples from 20 fertile women with negative, and from 6 infertile women with positive serum‐sperm agglutinating activity were subjected to a qualitative protein analysis by the Ouchterlony‐technique, to polyacrylamide (PAA) discelectrophoresis and after purification on spermatozoa they were also run against rabbit antihuman serum in the Laurell immuno‐electrophoretic technique. By the Ouchterlony method the main serum protein fractions were shown to occur also in cervical mucus. No significant differences could be found in the protein pattern of samples from fertile and infertile females as detected by polyacrylamide (PAA) electrophoresis. In all samples a split protein fraction corresponding to serum albumin and a band preceding the protein fraction corresponding to serum prealbumin were observed. In 2 samples from infertile females it could be demonstrated that immunoglobulins occurring in the cervical mucus were directed towards antigenic sperm components.