Open AccessPurification of the “Pregnancy Zone” Protein
Author(s) -
Schoultz Bo,
Stigbrand Torgny
Publication year - 1973
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.3109/00016347309158490
Subject(s) - sephadex , isoelectric point , chromatography , centrifugation , size exclusion chromatography , polyacrylamide gel electrophoresis , medicine , polyacrylamide , sedimentation coefficient , electrophoresis , isoelectric focusing , pregnancy , stokes radius , chemistry , biochemistry , biology , microbiology and biotechnology , enzyme , genetics
. The “pregnancy zone” protein is a serum protein found in pregnancy and after administration of oral contraceptives. It has hitherto not been isolated and therefore practically nothing is known about its structure and function. Purification of this protein was achieved in three steps: 1) Gel filtration on Sephadex G‐200; 2) Chromatography on DEAE‐Sephadex; 3) Preparative polyacrylamide electrophoresis. After the third step a protein was obtained which was found to be pure judged by the behaviour in polyacrylamide electrophoresis and ultra‐centrifugation. The molecular weight was estimated to be 326000, the sedimentation coefficient 12 S and the isoelectric point 4.7.