Open AccessMicrobodies in Tissue Cultures from Human Cervical Carcinomas
Author(s) -
Kurz Laura
Publication year - 1970
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.3109/00016347009157510
Subject(s) - haematoxylin , eosin , microbody , pathology , staining , stroma , cervix , h&e stain , medicine , epithelium , biology , cancer , immunohistochemistry , catalase , oxidative stress
. Tissue from 35 patients with invasive squamouscell cancer of the cervix uteri at different clinical stages was transplanted intraperitoneally, in 300 diffusion chambers into 300 castrated and non‐castrated female rats. The chambers were removed within 10 days. The contents of the chambers were fixed in 5% formalin and subjected to the usual methods of histological preparation, with staining by haematoxylin‐eosin. Filters with tissue cultures were fixed in 95% ethanol and then stained with haematoxylin‐eosin. The cultures were found to contain growth of epithelial and stroma elements. The epithelial proliferation was found to be increased in the neighbourhood of the fibroblasts. The rate of proliferation did not depend on the clinical stage of the cancer. Microbodies or blebs were found around the cell nucleus in varying numbers. The greatest number was found in cancer from clinically advanced cases. The nature of these microbodies has not been clarified, but they are in the course of being investigated.