Open AccessAnalysis of aflatoxin B1 and aflatoxigenic mold in commercial poultry feeds in Tamil Nadu, India
Author(s) -
Sireesha Yeturi,
J. Vimal Keerthana,
G. Sarathchandra,
Rethinasamy Velazhahan
Publication year - 2017
Publication title -
journal of applied and natural science
Language(s) - English
Resource type - Journals
eISSN - 2231-5209
pISSN - 0974-9411
DOI - 10.31018/jans.v9i1.1213
Subject(s) - aflatoxin , aspergillus flavus , sterigmatocystin , poultry farming , contamination , mold , tamil , biology , microbiology and biotechnology , toxicology , mycotoxin , food science , veterinary medicine , botany , medicine , ecology , linguistics , philosophy
A total of 48 commercial poultry feed samples collected from different poultry feed manufactures in Tamil Nadu, India were examined for the contamination of aflatoxin B1 (AFB1) and Aspergillus flavus. AFB1 in the samples was estimated by sandwich ELISA and the presence of A. flavus was detected by Real-Time PCR assay. Real-Time PCR analysis using A. flavus- specific omt primers confirmed the presence of A. flavus in all the samples tested. ELISA results indicated that the AFB1 contents in the poultry feeds ranged from 1.0 to18.7 ppb, which were below the permissible safe limits for poultry bird consumption and health. The results suggest adoption of good man-ufacturing practices by the commercial poultry feed manufacturers during procurement of feed ingredients, handling, storage and processing which might have suppressed the growth of A. flavus and aflatoxin contamination.