Open AccessIsolation of endophytic actinomycetes from Syzygium cumini and their antimicrobial activity against human pathogens
Author(s) -
Preeti Saini,
Madhurama Gangwar,
Anu Kalia,
Narinder Singh,
Deepti Narang
Publication year - 2016
Publication title -
journal of applied and natural science
Language(s) - English
Resource type - Journals
eISSN - 2231-5209
pISSN - 0974-9411
DOI - 10.31018/jans.v8i1.809
Subject(s) - syzygium , antimicrobial , chitinase , chitin , microbiology and biotechnology , isolation (microbiology) , plant use of endophytic fungi in defense , chloramphenicol , antibacterial activity , biology , staphylococcus aureus , chemistry , antibiotics , traditional medicine , bacteria , botany , enzyme , biochemistry , chitosan , medicine , genetics
Isolation of endophytic actinomycetes is an important step to screen antimicrobial compounds to curb the threat of drug-resistant strains of human pathogens. Out of the 50 endophytic actinomycetes obtained from surface sterilized root, stem and leaf tissues of Syzygium cumini, 50 isolates (30%) exhibited antimicrobial activity. Antistaphylococcal activity was displayed by most of the isolates, with maximum percent inhibition by J-10 (Mean of Inhibition Factor=12.12 mm2). A total of 8 isolates (4 each) were able to hydrolyse protein (proteinase activity) and solubilize chitin (chitinase activity). Results of thin layer chromatography confirm the production of chloramphenicol family |antibiotic by the isolate J-5. This is the first report providing an insight into untapped endophytic actinomycete milieu of Syzygium cumini yet to be explored which might be a promising source for novel antimicrobial agents.