Open AccessTriazophos induced neuro-splenic toxicity and evaluation of antioxidative potential of aqueous Broccoli extract in Wistar albino rats
Author(s) -
Dharmender Sharma,
Gurinder Kaur Sangha
Publication year - 2021
Publication title -
journal of applied and natural science
Language(s) - English
Resource type - Journals
eISSN - 2231-5209
pISSN - 0974-9411
DOI - 10.31018/jans.v13i2.2644
Subject(s) - glutathione , superoxide dismutase , oxidative stress , white pulp , glutathione peroxidase , chemistry , spleen , lipid peroxidation , glutathione reductase , toxicity , red pulp , antioxidant , catalase , pharmacology , endocrinology , medicine , biochemistry , enzyme , organic chemistry
The present investigation was carried out to assess the antioxidative potential of Broccoli sprouts aqueous extract (BE) against triazophos (TZ) induced oxidative stress (OS) in brain and spleen. In the experimental setup, six groups of rats were formed; Control (group 1), BE (group 2), TZ (group 3), and also BE+TZ groups such as BE1 (group 4), BE2 (group 5) and BE3 (group 6) groups. Rats were orally intubated for 30 days as per experimental design. After sacrifice, OS biomarkers viz; catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and lipid peroxidation (LPO) levels were determined in brain and spleen. Acetylcholinesterase (AChE) activity was observed in plasma and brain samples. Histological study of the spleen in TZ rats showed increased thickness of capsule, congestion and hypocellularity in follicles of spleen’s white pulp and the histoarchitecture was restored in TZ+BE group rats. TZ caused degenerative changes in brain histology and rats showed mild congestion along with haemorrhage in the cerebral cortex. Results suggest that TZ exposure is associated with neural toxicity along with altered spleen stress biomarkers, which further corroborates with histopathological findings. It is inferred that BE exerts multi-mechanistic protective effects against TZ induced neuro-splenic toxicity which is attributable to its protective antioxidant actions.