Open AccessTRANSCRIPTOME ANALASYS OF ADULT GULL-TAPEWORM DIBOTHRIOCHEPHALLUS DENDRITICUS (CESTODA)
Author(s) -
Сидорова,
Kutyrev,
Khabudaev,
Sukhanova,
Дугаров,
Zhepkholova,
Mazur Mazur
Publication year - 2020
Publication title -
theory and practice of parasitic disease control
Language(s) - English
DOI - 10.31016/978-5-9902341-5-4.2020.21.388-396
Subject(s) - transcriptome , biology , computational biology , cestoda , gene , identification (biology) , genetics , zoology , gene expression , ecology , helminths
In certain regions of Siberia and Baikal area, the main causative agent of diphyllobothriasis of human and animals is gull-tipeworm Dibothriochephallus dendriticus. In last decade, there was heightened interest to the study of molecules secreted by helminth which can modulate the immune response of their mammal hosts, particularly human. Bioinformatic analysis of transcriptomes gives opportunity to obtain data on proteins synthetized in certain time point and find among them ones the most significant for immunological functions. The aim of our study was to carry out transcriptome analysis of adult D. dendriticus. Adult D. dendriticus were pulled from the gut of herring gull. Total RNA was extracted with TRIzol reagent. Bar-coding transcriptome libraries were created. Sequencing obtained libraries was carried out using high-performance sequencer Illumina NextSeq550. De-novo assembly of transcriptome was performed. Annotation of obtained transcripts was done with Blast2Go software. Annotation allowed to describe their classification in terms of biological processes, molecular functions, and cellular components. At the next step, we plan to use D. dendriticus, assembled and annotated with transcriptome of plerocercoids, to search genes, potentially coding proteins that have an immunomodulatory effect on their hosts and also for identification of the proteins in secretome of parasites.