Open AccessCOMPARATIVE EFFICIENCY OF OVOCID DRUG BINGSTI AND PHENOL IN TEST IN VITRO
Author(s) -
Р Т Сафиуллин,
Shibitov,
Е. О. Качанова
Publication year - 2020
Publication title -
theory and practice of parasitic disease control
Language(s) - English
DOI - 10.31016/978-5-9902341-5-4.2020.21.368-374
Subject(s) - toxocara canis , biology , canis , veterinary medicine , larva , livestock , saline , toxicology , botany , ecology , medicine , endocrinology
It was determined that external environment objects are contaminated by infective elements at livestock farms. Despite animal epidemic countermeasures, complete prevention of parasitosis is not achieved at livestock farms, and new drugs for disinvasion appear but not all of them meet the standards of effectiveness against exogenous stages of helminths and other parasites. The ovocidal efficacy of the recommended concentration of Bingsti compared to base drug Phenol was studied in a test in vitro after cultivation of Toxocara canis eggs in Petri dishes. 500 eggs were placed in each chamber. The first chamber was a control one in which cultivation took place in normal saline. The recommended concentration of Bingsti with the 24-hour exposure was placed into the second chamber. A 4% solution of base drug Phenol with the 24-hour exposure was placed into the third chamber. T. canis eggs were investigated microscopically upon exposure, and then cultivated. During cultivation, the aeration was carried out, and embryogenesis was observed. Egg viability was determined by their appearance under the light microscopy by staining.The observations during the cultivation period showed that blastomeres turned into a larva on the 5th to 7th days after the cultivation start in the first (control) and second (Bingsti) groups; the larvae were motile until 10th-15th days, then they were at rest and became mobile only when heated. Blastomeres in T. canis eggs did not develop throughout the entire cultivation in the third group (phenol). The survival of T. canis eggs during cultivation was assessed by counting 100 eggs from each group under a microscope. There were 2% dead eggs in the first group (normal saline), 4% in the second group (Bingsti) and 100% in the third group (Phenol). The staining showed that live eggs were not stained from the first (control) and second (Bingsti) groups, and the embryos of dead eggs were colored blue from the third group (Phenol).