Open AccessUSE OF MALDI-TOF MS METHOD FOR SPECIES DIFFERENTIATION OF NEMATODES (BY EXAMPLE OF STUDYING PROTEIN PROFILES OF ASCARIDATE AND DIROFILARIA)
Author(s) -
Nagorniy,
A. Aleshukina,
I. Aleshukina,
Ermakova
Publication year - 2020
Publication title -
theory and practice of parasitic disease control
Language(s) - English
DOI - 10.31016/978-5-9902341-5-4.2020.21.267-275
Subject(s) - ascaris suum , nematode , dirofilaria , biology , dirofilaria immitis , chemistry , zoology , helminths , ecology
The article presents the results of studying protein profiles of nematodes relevant for the south of Russia (dirofilaria and ascaridate) using proteomic analysis based on MALDI-TOF MS. Analysis of the protein extracts of dirofilaria and ascaridate showed spectra with high-intensity peaks in the range of 2–20 kDa, the quality of the spectra and the intensities of the spectral peaks were consistent for all samples of the same species. It was noted that reliably frequent peaks were observed in the spectra obtained from various dirofilariae (D. repens and D. immitis) in the range from 3400 to 6000 kDa, that characterize the entire genus of nematodes. In the range above 6000 kDa, the spectra differed by representative peaks, which allowed us to differentiate one species from another according to the protein profile. The spectral profiles obtained from the ascarid A. suum and A. lumbricoi proteins, unlike dirofilaria, showed almost complete homology of graphic images in the m/z range from 3000 kDa to 15 000 kDa. At the same time, protein peaks in the range of 3000–7300 kDa, typical for the entire genus Ascaris, are constant. Three main peaks in both species were found in m/z 13 000, 13 400 and 14 400. Thus, the method of proteomic analysis based on MALDI-TOFF MS can serve as an effective tool in studies of the taxonomic identification of the pathogen.