Open AccessEFFECT OF BACTERIAL BIONEMATICIDES ON THE VIABILITY OF LARVAE MELOIDOGYNE
Author(s) -
Konrat
Publication year - 2020
Publication title -
theory and practice of parasitic disease control
Language(s) - English
DOI - 10.31016/978-5-9902341-5-4.2020.21.142-146
Subject(s) - gall , larva , nematode , bacillus (shape) , biology , suspension (topology) , bacteria , microbiology and biotechnology , biological pest control , suspension culture , bacillus thuringiensis , strain (injury) , botany , ecology , anatomy , cell culture , genetics , mathematics , homotopy , pure mathematics
The aim of this study was to establish the nematicidal effect of bacterial suspension of the Bacillus BZR 86 strain on the larvae of gall nematodes of the genus Meloidogyne spp. at different concentrations of bacterial culture. The experiment had 5 options and control. 1 ml of larval suspension and 1 ml of bacterial suspension were added to the tablet cells. The tablet cells were covered with a glass plate and stored at a temperature of 25 0C, for 24, 48, 72 hours. During these periods, the number of mobile and non-mobile larvae in each cell was counted under a binocular magnifying glass. After 48 hours, they were washed with sterile tap water. After passing 24 hours after washing, the number of living and dead nematodes was counted. When studying the effect of bionematicide on the viability of gall nematode larvae, it was found that for an undiluted suspension of Bacillus BZR 86, the mortality of larvae was 97.5%, when diluted five times 89%, ten times 64%, when diluted 100 and 1000 times, and in control, the larvae retained viability. The nematicidal activity of the studied Bacillus BZR 86 strain is high and reaches 64–97.5%. We can conclude that Bacillus BZR 86 can be the basis for creating a biological drug to reduce the harmfulness of gall nematodes.