Open AccessMETHOD OF FORMALIN-ETHER SEDIMENTATION IN THE STUDY OF NATURAL FOCI OF OPISTHORCHIASIS
Author(s) -
Ушаков
Publication year - 2019
Language(s) - English
DOI - 10.31016/978-5-9902340-8-6.2019.20.639-644
Subject(s) - opisthorchiasis , preservative , sedimentation , feces , veterinary medicine , sediment , tributary , biology , environmental chemistry , environmental science , chemistry , microbiology and biotechnology , food science , geography , medicine , pathology , paleontology , cartography
Of all the tested coproovoscopic research methods used in medical and veterinary practice, the most optimal for the study of natural foci of opisthorchiasis is the meth-od of formalin-ether sedimentation. Its advantage is in using Barbagallo solution as a preservative, which allows this method to process the material collected in the foci of trematodoses, without any intermediate operations. The great advantage of the method is the purity of the studied sediment and the ability to detect even single helminth eggs. The use of preservative allows storing the material without providing any special conditions for a long time (1-2 years). The studies were carried out in the ecosystems of the Ob, Irtysh, Tobol, Konda, Kuma, Yukonda and their tributaries. Rodent excrement (not less than 2 grams), not in contact with the alluvial deposits of the riverbed and floodplain, were selected in penicillin bottles of 20 ml with a preservative – Barbagallo solution. 4 074 samples of excrement of water voles were investigated by the method of formalin-ether sedimentation. Eggs of trematodes of the Opisthorchidae family were found in 546 samples of rodent excrement. From 1 to 67 eggs of trematodes were detected in each sample.