Open AccessINTESTINAL MICROBIOME BY HISTOCOPROLOGICAL ANALYSIS AND ITS CLINICAL SIGNIFICANCE
Author(s) -
Chernysheva,
Babaeva,
Yurieva
Publication year - 2021
Publication title -
theory and practice of parasitic disease control
Language(s) - English
DOI - 10.31016/978-5-6046256-1-3.2021.22.552-559
Subject(s) - antibiotics , bloating , feces , diarrhea , microbiome , abdominal pain , dose , constipation , biology , helminthiasis , deworming , medicine , microbiology and biotechnology , gastroenterology , helminths , veterinary medicine , immunology , bioinformatics
The histocoprological method was used to study stool samples from patients with gastrointestinal diseases to determine the presence of helminths and assess the stateof the intestinal microbiome. The main complaints of patients were abdominal pain,bloating, constipation and diarrhea. The patients were divided into two groups: thosewho took antibiotics and those who did not. Evaluation of the microbial backgroundby histological stool preparations was carried out in total, morphologically by thedensity of the location of microbial colonies and the intensity of their color. Helminthiasis was ascertained by the detection of fragments of small nematodes, larvae, their cuticles and eggs. A high percentage of depletion of the microbial background for drugs with a decrease in the number of microbial colonies was noted in the first group (65.1% of those who took antibiotics). Among children, a weakening of the microbiome was found in 42 (88% – who also took antibiotics), that is, more often.The negative effect of antibiotics and other pathogens on the microbial backgroundrequired its correction with the appointment of sorbents and prebiotics. All examined patients were found to have helminthiases (nematodes and mixed forms). Deworming was carried out with nemazole at age-specific dosages. In 57 patients who re-passed the analysis, after complex treatment, there was a positive trend, clearly traced in feces preparations.