Open AccessTuberculins for mammals, stability of biological properties of old Koch altuber-culin (Germany) after long-term storage
Author(s) -
A.H. Najmanov,
Yu.M. Мyasoedov
Publication year - 2021
Publication title -
veterinariâ i kormlenie
Language(s) - English
Resource type - Journals
ISSN - 1814-9588
DOI - 10.30917/att-vk-1814-9588-2021-2-7
Subject(s) - tuberculin , reactogenicity , immunology , medicine , mycobacterium bovis , tuberculin test , biology , tuberculosis , pathology , immune system , mycobacterium tuberculosis , immunogenicity
The allergic method is the main one in the diagnosis of mycobacterial infections in humans and animals. Allergic diagnosis of mycobacterial infections is carried out us-ing tuberculin. Tuberculin was developed over a hundred years ago by the German microbiologist Robert Koch. The diagnostic properties of tuberculin have been studied in detail. This made it possible to develop various methods of using tuberculin in a clinical setting. In addition, tuberculin is still used in the study of the fundamental mechanisms of immunity. Until now, tuberculin is a valuable diagnostic tool. However, the stability of tu-berculin over a long period of time has not been studied. The article provides a historical overview of the manufacture and use of various types of tuberculins for mammals, as well as the results of our own research. Purpose of the study: to study the stability of the biological properties of altuberculin for mammals (Germany) after long-term storage. The study of stability was carried out according to the following parameters: activity, specificity, reactogenicity. The activity was studied in guinea pigs sensitized with M. bovis mycobacteria. The specificity was investigated in guinea pigs sensitized with M. avium mycobacteria. Reactogenicity was studied in non-sensitized guinea pigs. As a result of the research, it was found that alttuberculin for mammals is characterized by biological activity at the level of 8000 IU in accordance with the current active standard of activity (the value of biological activity should correspond to the range of 6600 IU - 15000 IU). Altuberculin is characterized by a low specificity at the level of 41% (the specificity value should not be more than 10%). Alttuberculin, in accordance with modern requirements for the quality of purified tuberculins, is characterized by a high level of reactogenicity (the value of reactogenicity is expressed in the development of inflammation, the size of which should not exceed 5 mm). Thus, Alttuberculin for mammals maintains the stability of biological properties with a shelf life of more than 70 years. A comparative analysis of the quality indicators of alttuberculin and purified tuberculin PPD indicates the preference of using the purified form of the allergen when conducting diagnostic studies of animals for tuberculosis.