Open AccessMethods of genome editing for increasing the shelf life of tomato fruit
Author(s) -
Ю. В. Кузьмина
Publication year - 2020
Publication title -
biotehnologiâ i selekciâ rastenij
Language(s) - English
Resource type - Journals
eISSN - 2658-6266
pISSN - 2658-6258
DOI - 10.30901/2658-6266-2020-1-o6
Subject(s) - genome editing , transcription activator like effector nuclease , zinc finger nuclease , crispr , cas9 , genome , biology , computational biology , effector , palindrome , genetics , gene , microbiology and biotechnology
Genome editing methods are now widely used in research aimed at studying fundamental biological processes, in particular for regulating maturation and extending shelf life of plant agricultural products. This review briefly discusses plant genome editing methods and examples of their successful application for increasing the storage life of fruits of tomato as one of the most important crops. Genome editing is one of the new areas of genetic engineering that is truly revolutionary in biotechnology. Various genome editing systems have been developed over the past decades: zinc finger nucleases (ZFNs), transcriptional activator-like effector nucleases (TALENs), and clustered regularly located short palindromic repeats recognized by Cas9 nuclease (CRISPR/Cas9). The most common and widely used is the CRISPR/ Cas9 system, which has many advantages over other existing genome editing systems.