Open AccessEfficiency of SSR and PawS markers for evaluation of genetic polymorphism among red clover (Trifolium pratense L.) cultivars
Author(s) -
Ирина Клименко,
Sergey Kostenko,
Ю. М. Мавлютов,
А. О. Шамустакимова
Publication year - 2020
Publication title -
trudy po prikladnoj botanike, genetike i selekcii
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 2
eISSN - 2619-0982
pISSN - 2227-8834
DOI - 10.30901/2227-8834-2020-3-100-109
Subject(s) - cultivar , biology , amplicon , microsatellite , germplasm , red clover , genetic marker , genetic diversity , genotyping , plant breeding , horticulture , genetics , polymerase chain reaction , genotype , gene , allele , population , demography , sociology
Background. Identification of crop varieties is presently one of the most important aspects due a significant annual increase in the number of newly developed cultivars. Application of molecular markers makes it possible to identify cultivars and secure protection of plant breeders’ rights. Marker techniques based on SSR loci and PawS markers were evaluated for their efficiency in revealing the DNA polymorphism in Russian red clover cultivars, and the research results are presented in this publication. Materials and Methods . The total genome DNA was extracted by a modified SDS method from 30 seedlings per each cultivar. Nine simple sequence repeats (SSR) and 4 PawS markers were used for genotyping. The basic genetic diversity parameters were measured and analyzed using the software resources GelAnalyzer 2010а, MStools v.3, and Statistica 7.0. Results and conclusion . The mean level of intervarietal DNA polymorphism in red clover was 38.6%. Cultivar-specific amplicons were obtained for 4 accessions (cvs. ‘Trifon’, ‘Topaz’, ‘Trio’ and ‘Mars’) with SSR loci RCS1307 and RCS3095. These loci were found appropriate for identification and certification of such cultivars. The tested PawS markers (individually and in combinations) proved non-informative for the analysis of intervarietal DNA polymorphism in red clover. The only primer pair PawS5+PawS16 generated reproducible PCR products, but unique amplicons were absent in the DNA profiles. The data obtained in this study may be helpful for further identification and certification of Russian red clover cultivars and promising breeding materials.