Open AccessCalculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software
Author(s) -
П. В. Шадрин,
Т. А. Батуашвили,
Л. В. Симутенко,
Н. П. Неугодова
Publication year - 2021
Publication title -
vedomosti naučnogo centra èkspertizy sredstv medicinskogo primeneniâ
Language(s) - English
Resource type - Journals
eISSN - 2619-1172
pISSN - 1991-2919
DOI - 10.30895/1991-2919-2021-11-2-135-142
Subject(s) - effective dose (radiation) , median lethal dose , lethal dose , biometrics , algorithm , mathematics , toxicology , medicine , computer science , nuclear medicine , toxicity , biology , artificial intelligence
The median lethal dose (LD 50 ) and low lethal dose (LD 10 ) are calculated in acute toxicity studies, as well as during specific activity assessment of some medicines. The aim of the study was to develop a procedure for using CombiStats to calculate LD 50 and LD 10 . The authors proposed a step-by-step algorithm for processing bioassay results using the CombiStats biometric software (median effective dose determination model, probit analysis) with conversion of doses to simple fractions (fractions of the maximum dose) to calculate LD 50 and LD 10 . They compared LD 50 and LD 10 calculation results obtained using CombiStats with those obtained using electronic spreadsheets according to the Bliss–Miller–Tainter–Prozorovsky method described in the State Pharmacopoeia of the Russian Federation (General Monograph 1.1.0014.15). It has been demonstrated that the use of CombiStats sometimes has advantages over the use of the pharmacoepoeial method.