Open AccessPhylogenetic analysis shows that New Zealand isolates of Neonectria ditissima are similar to European isolates
Author(s) -
K.R. Everett,
I.P.S. Pushparajah,
R.W.A. Scheper
Publication year - 2021
Publication title -
proceedings of the new zealand weed and pest control conference/new zealand plant protection/proceedings of the ... national weeds conference/proceedings of the new zealand weed control conference/proceedings of the new zealand plant protection conference
Language(s) - English
Resource type - Journals
eISSN - 0370-2804
pISSN - 0370-0968
DOI - 10.30843/nzpp.2021.74.11747
Subject(s) - biology , phylogenetic tree , clade , polymerase chain reaction , internal transcribed spacer , canker , phylogenetics , genetics , ribosomal dna , gene , ribosomal rna , homology (biology) , botany
Neonectria ditissima causes a debilitating apple tree canker disease. We determined the efficacy of polymerase chain reaction primers, originally designed for European strains, by sequencing New Zealand strains. The concatenated ribosomal inter-transcribed spacer and β-tubulin gene regions of 17 New Zealand isolates were compared with those of two European strains by phylogenetic analysis. New Zealand and European isolates of N. ditissima were in the same clade, suggesting that there has been little change in these gene regions following introduction to New Zealand. There was 100% homology with Bt-FW135 and Bt-RW284 primers. Based on sequencing 17 New Zealand isolates from several locations, these polymerase chain reaction primers can be relied upon to amplify New Zealand isolates of N. ditissima.