Time and temperature combinations that are lethal to Pseudomonas syringae pv actinidiae

The thermal death point was determined in vitro for three isolates of Pseudomonas syringae pv actinidiae (Psa) A bacterial suspension was treated by placing in an Eppendorf tube then floating in a water bath at seven temperatures from 263 to 60C At each temperature an aliquot was removed after 5 15 35 75 155 315 and 635 min placed onto Kings medium B (KMB) in Petri plates and spread with a sterilised bent glass rod Petri plates were placed at 25C for 24 h and colonies counted There were three isolate replicates each in an Eppendorf tube per timetemperature combination There was no difference in response between isolate replicates but time and temperature differences were significant (P