Open AccessDetecting a Tospovirus in trapped thrips
Author(s) -
R.A. Lister,
M-C. Nielsen,
M.M. Davidson,
J. D. Fletcher,
Gail M. Timmerman-Vaughan
Publication year - 2009
Publication title -
proceedings of the new zealand weed and pest control conference/new zealand plant protection/proceedings of the ... national weeds conference/proceedings of the new zealand weed control conference/proceedings of the new zealand plant protection conference
Language(s) - English
Resource type - Journals
eISSN - 0370-2804
pISSN - 0370-0968
DOI - 10.30843/nzpp.2009.62.4857
Subject(s) - tospovirus , thrips , biology , bunyaviridae , tomato spotted wilt virus , vector (molecular biology) , western flower thrips , virology , plant virus , thripidae , botany , virus , genetics , gene , recombinant dna
Thrips vector tospoviruses which worldwide cause disease epidemics in a wide range of economically important crops Of the approximately 5000 species of thrips 11 are known to vector one or more of the 14 recognised Tospovirus species In New Zealand only three tospoviruses and two vectors have been recorded Frankliniella occidentalis and Thrips tabaci vector tomato spotted wilt virus (TSWV) while T tabaci vectors iris yellow spot virus (IYSV) and impatiens necrotic spot virus (INSV) is vectored primarily by F occidentalis Frankliniella occidentalis is also known to vector three other tospoviruses not yet recorded in New Zealand It was possible to detect the presence of a tospovirus (TSWV) from thrips (F occidentalis) trapped on sticky boards for 24 h and 1 2 3 or 4 weeks using ELISA and RTPCR techniques ELISA provides a rapid and economical screening technique provided antibodies are commercially available for a given tospovirus However the use of RTPCR may allow the development and use of universal primers to detect the presence of any Tospovirus which could then be further identified using specific Tospovirus primers