Affinity Precipitation Of Enzyme By Using Conjugated Ligand-Poly(Nipam) Polymer | Zendy

Syaubari Syaubari | Zendy; Markus Saari | Zendy; Chuah T.G | Zendy; G. Zulkafli | Zendy; M. Ishak | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Affinity Precipitation Of Enzyme By Using Conjugated Ligand-Poly(Nipam) Polymer

Author(s) -

Syaubari Syaubari,

Markus Saari,

Chuah T.G,

G. Zulkafli,

M. Ishak

Publication year - 2016

Publication title -

jurnal sains dan teknologi reaksi

Language(s) - English

Resource type - Journals

eISSN - 2549-1202

pISSN - 1693-248X

DOI - 10.30811/jstr.v3i2.51

Subject(s) - polymer , trypsin , ligand (biochemistry) , conjugated system , chemistry , precipitation , polymer chemistry , enzyme , affinity chromatography , organic chemistry , biochemistry , receptor , physics , meteorology

In this paper, preparation of N-isopropylacrylamide of varying transfer chain lengths was synthesized. p-Aminobenzamidine (PABA) was chemically linked to the carboxyl groups of these polymers to obtain thermoprecipitating affinity polymers. The polymer to which PABA was linked through a carboxyl groups exhibited increase with polymer containing various PABA. Investigations on experiment showed that this enhancement in trypsin binding by the polymers was due to the p-aminobenzamidines to microenvironmental effects. Recovery and specific activity of the trypsin recovered increased with the low molecular weight of polymer.Keywords: Ligand, p-aminobenzamidine, trypsin, recovery, affinity precipitation

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research