Open AccessAffinity Precipitation Of Enzyme By Using Conjugated Ligand-Poly(Nipam) Polymer
Author(s) -
Syaubari Syaubari,
Mohd Yusof Saari,
Chuah T.G,
G. Zulkafli,
M. Ishak
Publication year - 2016
Publication title -
jurnal sains dan teknologi reaksi/jurnal sains dan teknologi reaksi
Language(s) - English
Resource type - Journals
eISSN - 2549-1202
pISSN - 1693-248X
DOI - 10.30811/jstr.v3i2.51
Subject(s) - polymer , trypsin , ligand (biochemistry) , conjugated system , chemistry , precipitation , polymer chemistry , enzyme , affinity chromatography , organic chemistry , biochemistry , receptor , physics , meteorology
In this paper, preparation of N-isopropylacrylamide of varying transfer chain lengths was synthesized. p-Aminobenzamidine (PABA) was chemically linked to the carboxyl groups of these polymers to obtain thermoprecipitating affinity polymers. The polymer to which PABA was linked through a carboxyl groups exhibited increase with polymer containing various PABA. Investigations on experiment showed that this enhancement in trypsin binding by the polymers was due to the p-aminobenzamidines to microenvironmental effects. Recovery and specific activity of the trypsin recovered increased with the low molecular weight of polymer.Keywords: Ligand, p-aminobenzamidine, trypsin, recovery, affinity precipitation