Open AccessAcute Normovolemic Hemodilution-assisted Terminal Blood Procurement in Swine for Ex Vivo Organ Perfusion
Author(s) -
Wei Wu,
John W. Stokes,
Rei Ukita,
Ioannis A. Ziogas,
Yatrik J Patel,
Sophoclis P. Alexopoulos,
Matthew Bacchetta,
Clayne Benson
Publication year - 2022
Publication title -
journal of the american association for laboratory animal science
Language(s) - English
Resource type - Journals
eISSN - 2769-6677
pISSN - 1559-6109
DOI - 10.30802/aalas-jaalas-21-000074
Subject(s) - perfusion , medicine , ex vivo , blood volume , hemoglobin , anesthesia , organ procurement , whole blood , hematocrit , femoral artery , in vivo , surgery , cardiology , biology , microbiology and biotechnology , transplantation
Swine ( Sus scrofa domesticus ) are commonly used large animal subjects for the study of disease and preclinical therapies. Organ machine perfusion is a therapy that has gained momentum as a research platform for the study of ex vivo organ preservation and therapeutics. However, complex perfusion circuits and research protocols often require large volumes of blood as perfusate. Here, we report a technique for increasing terminal blood yield during swine organ and blood procurement; our method involves acute normovolemic hemodilution and exsanguination via the femoral artery. We collected a total of 47 ± 4 mL/kg of blood and 4.3 ± 0.6 g/kg of hemoglobin, representing 73% ± 6% of the estimated blood volume and 64% ± 8% of the total estimated intravascular hemoglobin ( n = 4). Neither pH, lactate, nor pO2 levels changed significantly during blood procurement. Acute normovolemic hemodilution is an effective method for increasing RBC and hemoglobin yield during blood procurement in swine.