Open AccessOptimization of the extraction conditions for Chlorophyll a from fresh Spirulina
Author(s) -
Nguyễn Thị Thảo,
Ta Thac Binh,
Pham Thi Khanh Linh,
Vũ Hồng Sơn,
Nguyễn Thị Minh Tú
Publication year - 2022
Publication title -
food science and applied biotechnology
Language(s) - English
Resource type - Journals
ISSN - 2603-3380
DOI - 10.30721/fsab2022.v5.i1.149
Subject(s) - spirulina (dietary supplement) , solvent , extraction (chemistry) , chlorophyll a , chlorophyll , cyanobacteria , chemistry , algae , chromatography , botany , nuclear chemistry , biology , biochemistry , raw material , organic chemistry , bacteria , genetics
Spirulina is a spiral cyanobacteria with simple cell structure, making it easier to extract chlorophyll than other materials. Investigation the ultrasound assisted extraction of chlorophyll a was conducted on the following factors: type of solvent, concentration of solvent, ratio of solvent: material, time and temperature. Optimization by Design Expert the extraction of chlorophyll a from spirulina algae indicates the condition to obtain the highest amount of chlorophyll a of 15.07 mg/g was as followings: solvent ethanol concentration 99.7%, ratio solvent / material: 16/1, time 2.5 hours, temperature 52°C, ultrasonic frequency 40kHz.