Optimization of the extraction conditions for Chlorophyll a from fresh Spirulina | Zendy

Nguyễn Thị Thảo | Zendy; Ta Thac Binh | Zendy; Pham Thi Khanh Linh | Zendy; Vũ Hồng Sơn | Zendy; Nguyễn Thị Minh Tú | Zendy

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Optimization of the extraction conditions for Chlorophyll a from fresh Spirulina

Author(s) -

Nguyễn Thị Thảo,

Ta Thac Binh,

Pham Thi Khanh Linh,

Vũ Hồng Sơn,

Nguyễn Thị Minh Tú

Publication year - 2022

Publication title -

food science and applied biotechnology

Language(s) - English

Resource type - Journals

ISSN - 2603-3380

DOI - 10.30721/fsab2022.v5.i1.149

Subject(s) - spirulina (dietary supplement) , solvent , extraction (chemistry) , chlorophyll a , chlorophyll , cyanobacteria , chemistry , algae , chromatography , botany , nuclear chemistry , biology , biochemistry , raw material , organic chemistry , bacteria , genetics

Spirulina is a spiral cyanobacteria with simple cell structure, making it easier to extract chlorophyll than other materials. Investigation the ultrasound assisted extraction of chlorophyll a was conducted on the following factors: type of solvent, concentration of solvent, ratio of solvent: material, time and temperature. Optimization by Design Expert the extraction of chlorophyll a from spirulina algae indicates the condition to obtain the highest amount of chlorophyll a of 15.07 mg/g was as followings: solvent ethanol concentration 99.7%, ratio solvent / material: 16/1, time 2.5 hours, temperature 52°C, ultrasonic frequency 40kHz.

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