Open AccessEffects of genetic variation in the 20th intron of Sansui duck ATP2A2 gene on eggshell quality
Author(s) -
Junhan Mei,
Yiyu Zhang,
Minfang You,
Chao-Mei Liao,
Genjia Tan,
Jiezhang Li
Publication year - 2021
Publication title -
journal of zoological research
Language(s) - English
Resource type - Journals
ISSN - 2630-5100
DOI - 10.30564/jzr.v3i2.3048
Subject(s) - eggshell , intron , biology , single nucleotide polymorphism , linkage disequilibrium , genetics , genotype , snp , gene , ecology
In order to explore the influence of the polymorphism in the 20 intron region of the Sansui duck ATP2A2 gene on the eggshell quality, this study used Primer Premier 5 software to design and synthesize a pair of primers in the 20 intron region, two-way direct sequencing and sequence alignment to mine SNPs Sites, SPSS 18.0 software was used to analyze the relationship between SNP sites and eggshell quality of Sansui duck. Results Three SNP sites were found in the 20 intron region of the ATP2A2 gene: g.40874 T>C, g.40920 G>A and g.40990 T=C, all of which were moderately polymorphic, at the site g.40874 T C and g.40920 GA both deviated significantly from Hardy-Weinberg equilibrium (P>0.05), position g.40990 T=C accords with Hardy-Weinberg equilibrium (P C and g.40990 T=C found in the 20th intron region of the Sansui duck ATP2A2 gene may be the marker sites that affect the quality of the eggshell.