Open AccessDistribution of E.coli O157:H7 in fecal and urine samples of cattle
Author(s) -
Aseel M H Al-Rudha,
Ezdihar Mohammed Mahal Al-Rubaie,
Nuha Khalaf Khalil
Publication year - 2016
Publication title -
the iraqi journal of veterinary medicine/al-maǧallaẗ al-ṭibbiyyaẗ al-bayṭariyyaẗ al-’irāqiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2410-7409
pISSN - 1609-5693
DOI - 10.30539/iraqijvm.v40i1.142
Subject(s) - feces , cefixime , urine , biology , veterinary medicine , agar , microbiology and biotechnology , zoology , medicine , bacteria , antibiotics , cephalosporin , biochemistry , genetics
This study was designed to isolate of Enterohaemoragic E.coli O157:H7 from feces and urine in cattle. 175 samples (80 fecal and 95 urine) were collected to isolate EHEC O157:H7 during the period from March to June 2014 from Al- Hindia slaughter house from local breeding cattle, aged between 1-2 years and over 2 years, and the samples were subjected to culture on specific media Cefixime Tellurit- Sorbitol MacConkey agar at 37 ˚C for 18-24 hrs. then the isolates were identified by biochemical tests (Indol test, Potassium cyanid test) and seriologically by Latex Kit test. The results showed that 73(91.25%) out of 80 fecal samples and 39(41%) out of 95 urine samples were E.coli O157:H7 positive, the total of positive isolates were 64%. That reveals the important role of feces and urine samples to contaminate the environment and disseminate of infection.