Open AccessEvaluating the bacterial culture technique by Polymerase chain reaction for the diagnosis of Brucella abortus in milk of cows suspected with brucellosis.
Author(s) -
Bashar Sadeq Noomy
Publication year - 2016
Publication title -
the iraqi journal of veterinary medicine/al-maǧallaẗ al-ṭibbiyyaẗ al-bayṭariyyaẗ al-’irāqiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2410-7409
pISSN - 1609-5693
DOI - 10.30539/iraqijvm.v40i1.130
Subject(s) - brucellosis , brucella abortus , brucella , brucellaceae , abortion , microbiological culture , biology , polymerase chain reaction , aborted fetus , veterinary medicine , brucella melitensis , sheep milk , bovine brucellosis , pcr test , microbiology and biotechnology , pregnancy , virology , medicine , bacteria , food science , biochemistry , genetics , gene
The aim of this study is to determine the sensitivity of bacterial culture technique in the detection of Brucella abortus in milk samples of aborted cows. Sixty samples of milk were collected from aborted cows during a period which did not exceed two months after the abortion. All of them were positive for rose bengal test. Results showed that Brucella abortus was isolated from 7 out of 60 (11.6%) from the milk of aborted cows, while PCR test showed that 32 out of 60 (53.3%) milk sample contained Brucella abortus. The specificity of culture techniques was 10%, but its sensitivity was only 21.8%. Beside the cautions in dealing with live Brucella abortus (as culture), it is also less sensitive than PCR, though it is better to use PCR technique in the diagnosis of brucellosis in aborted cows milk.