Detection of Brucella antibodies of sheep and goats by using two serological tests in Al-Sulaimanya governorate
Author(s) -
Osman M. Jabary
Publication year - 2015
Publication title -
the iraqi journal of veterinary medicine
Language(s) - English
Resource type - Journals
eISSN - 2410-7409
pISSN - 1609-5693
DOI - 10.30539/iraqijvm.v39i2.174
Subject(s) - flock , veterinary medicine , rose bengal , serology , brucellosis , brucella , biology , bengal , zoology , brucella melitensis , antibody , medicine , immunology , geography , genetics , archaeology , bay
A research was carried out to detect Brucella antibodies of sheep and goats in Al-Sulaimanya governorate by using modified Rose Bengal test and indirect ELISA. A total of three hundred and eleven (311) whole blood samples (160 sheep and 151 goats) were collected randomly from eight different regions in Al-Sulaimanya governorate from unvaccinated flock with different ages. A total percentages of positive result by using modified Rose Bengal test was 14.46% (20% in sheep and 8.6% in goats), while by using indirect ELISA was 27.6% (35.2% in sheep and 19.2% in goats) with significant (P<0.05) differences. It revealed that rates to modified Rose Bengal test were 14.34% in female and 10.09% in males while to indirect ELISA 26.35% in female and 33.9% in males in sheep and goats, In conclusion of this study the high seroprevelence was at 1-3 years 19.2% and ˃3 years 33.96% according to modified Rose Bengal test and indirect ELISA, respectively.
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