Open AccessThe Differences in Bioactivity between Crude and Purified Cholera Toxin from Local Isolate of Vibrio cholera
Author(s) -
Ami. Al. Thwani
Publication year - 2012
Publication title -
the iraqi journal of veterinary medicine/al-maǧallaẗ al-ṭibbiyyaẗ al-bayṭariyyaẗ al-’irāqiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2410-7409
pISSN - 1609-5693
DOI - 10.30539/iraqijvm.v36i0e.384
Subject(s) - toxin , cholera toxin , cholera , vibrio cholerae , spleen , microbiology and biotechnology , guinea pig , biology , chemistry , bacteria , immunology , endocrinology , genetics
In this investigate Cholera toxin was purified from the local clinical isolatedesignated as V. cholerae S . Few steps were employed for purification of CTincluding concentration of the protein, back extraction, and gel filtration. Thebioactivity of CT reflected that crude and purified toxin had lethal activity on Balb/cmice in which a dose of 2.5µg/ ml of purified toxin caused mice death withsymptoms such as muscle cramps and tachycardia while erythematous andinduration was seen at intradermal I. d injection of skin of Guinea pig.Histopathological examination for liver, intestine, adrenal gland and spleen of whitemice showed that liver tissue was more affected compared with spleen, intestine andadrenal gland tissues.