Open AccessEvaluation the efficacy of immunological response in sheep vaccinated with Brucella melitensis strain Rev 1
Author(s) -
Wisam S. Al Khafaji
Publication year - 2012
Publication title -
the iraqi journal of veterinary medicine/al-maǧallaẗ al-ṭibbiyyaẗ al-bayṭariyyaẗ al-’irāqiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2410-7409
pISSN - 1609-5693
DOI - 10.30539/iraqijvm.v36i0a.346
Subject(s) - brucella melitensis , titer , serology , vaccination , direct agglutination test , antibody , antibody response , medicine , rose bengal , antibody titer , immunology , brucella , biology , brucellosis , genetics
The present study aimed to identify the efficacy of Brucella melitensis vaccine strain Rev 1 in induction of antibodies by different doses and routes of administration; also to obtain the specificity of the serological tests, the study included 28 ewes divided into four equal groups . the animals of first and second groups vaccinated subcutaneously with 2×109 and 2×107 colony forming units (CFU) respectively while the animals of third and fourth groups vaccinated conjunctively with 2×109 and 2×107 CFU respectively . The sera were collected at zero time, 2, 4, 8, 16, 20, 24 and 28 weeks of the experiment and the antibodies response were evaluated using classical tests ( Rose Bengal, serum agglutination and 2-mercaptoethanol tests ) compared with competitive ELISA test , and brucellin test was used to detect the cellular response . The results showed that antibody titers were higher and remained for longer period in subcutaneously vaccinated groups in both doses compared with those vaccinated conjunctively and the 2-mercaptoethanol test show the best specificity in all vaccinated groups; also the subcutaneously vaccinated groups recorded significant increase in skin thickness in brucellin test after 12 week post vaccination .There was a significant increase in neutrophils activity for reduction of nitrobluetetrazolium stain in all groups between 2nd and 8th post vaccination