Open AccessCytotoxic Effect of Datura stramonium Extract on Cancer Cell lines
Author(s) -
Nahi Y. Yaseen,
Khalil Z. Al Jeboori,
Guido Ala,
Ala'a H. Merza
Publication year - 2010
Publication title -
the iraqi journal of veterinary medicine/al-maǧallaẗ al-ṭibbiyyaẗ al-bayṭariyyaẗ al-’irāqiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2410-7409
pISSN - 1609-5693
DOI - 10.30539/iraqijvm.v34i1.669
Subject(s) - datura stramonium , cytotoxic t cell , cell culture , cytotoxicity , viability assay , cancer cell , fibroblast , biology , datura , in vitro , cell , cancer , traditional medicine , microbiology and biotechnology , chemistry , botany , biochemistry , medicine , genetics
In order to investigate the in vitro antitumor activity of Datura stramonium seeds on cancer cell lines. Extract of this plant was prepared by using different concentrations of seed extract 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75 and 5 mg/ ml. Cytotoxicity was estimated on mammary adenocarcinoma (AMN3), brain cancer, and normal rat embryonic fibroblast (Ref3) cell lines. The results exhibited that the extract has cytotoxic effect by decreasing the viability of AMN3 (42.91%) and brain cell lines (32.79%). However, it produced little effect on viability of normal cell line Ref3, indicating the specificity of this extract against malignant cells