Open AccessEffect of Temperature on the Viability of Protoscolices of Echinococcus granulosus invitro
Author(s) -
Amall Hassan Atia
Publication year - 2008
Publication title -
the iraqi journal of veterinary medicine/al-maǧallaẗ al-ṭibbiyyaẗ al-bayṭariyyaẗ al-’irāqiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2410-7409
pISSN - 1609-5693
DOI - 10.30539/iraqijvm.v32i1.762
Subject(s) - echinococcus granulosus , eosin , biology , andrology , survival rate , vital stain , cell survival , in vitro , veterinary medicine , pathology , medicine , staining , zoology , biochemistry , genetics
A study was conducted invitro to determine the effects of storage attemperature 4 Cº upon the survival of protoscolices of Echinococcusgranulosus from 86 hydatid cysts isolated from infected donkeys . Flam cellactivity was the choice for viability , in addition using vital stain (0.1%aqueous eosin) . The result showed that higher percentage survival 94.41%in hydatid fluid for 9 days . The longest survival times of any of the samplestested was 81 days with the survival percentage 85% and the lowest was 33%for 13 days , showing movement till the end of this period . Our resultsproved the protoscolices removed from cyst retain their survival for severalweeks at refrigerator temperature when stored in hydatid fluid in test tubes .