Open AccessIsolation &Identification of Newcastle Disease Virus From Wild Pigeons
Author(s) -
Anton S. Al-Bana
Publication year - 2007
Publication title -
the iraqi journal of veterinary medicine/al-maǧallaẗ al-ṭibbiyyaẗ al-bayṭariyyaẗ al-’irāqiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2410-7409
pISSN - 1609-5693
DOI - 10.30539/iraqijvm.v31i2.796
Subject(s) - newcastle disease , isolation (microbiology) , virus isolation , virus , virology , inoculation , biology , microbiology and biotechnology , immunology
Isolation &identification of Newcastle disease virus from wild pigeonsinhabiting University of Baghdad (Al-Khwarizmi College of engineering)where velogenic and devastating infection of birds was encountered. Chickenembryos fibroblasts (CEF) were used for virus isolation (from infectedspleen and lungs) and propagation. Viral cytopathogenic effect was noticedon infected (CEF) cell culture within (24) hr post inoculation. The virus wasidentified by heamagglutination inhibition (HI) test using reference antiNDV serum.