Open AccessUsing Modified Anthraxin And Its Evaluation For Testing Cell Mediated Immunity And Studying Histopathological Changes In Lab Animals
Author(s) -
An' am J.L. Al-Jeboury
Publication year - 2007
Publication title -
the iraqi journal of veterinary medicine
Language(s) - English
Resource type - Journals
eISSN - 2410-7409
pISSN - 1609-5693
DOI - 10.30539/iraqijvm.v31i2.791
Subject(s) - dermis , bacillus anthracis , anthrax vaccines , erythema , immunity , delayed hypersensitivity , virulence , immunology , biology , antigen , pathology , medicine , immunization , immune system , biochemistry , bacteria , gene , genetics , dna vaccination
This study aims to apply a new procedure in preparation of anthraxin fromcell wall extracts of a virulent Bacillus anthracis strain 34F2 (Sterne).Anthraxin was evaluated for potential use as skin testing agent to measurecell-mediated immunity. Guinea pigs were immunized and boostedsubcutaneously with Sterne live veterinary anthrax vaccine. Two weeks afterthe booster dose, animals were skin tested with the antigen which recorded15.6mm as a mean of erythema and 2.5 mm difference of thickness ascompared to the control. Histological changes corresponded with the delayedtype of hypersensitivity (DTH) in which the changes involvedinfiltration of inflammatory cells particularly macrophages, neutrophiles andeosinophils in the skin dermis and in perivascular region in addition toaccumulation of fluid (edema) in the dermis and congestion of blood vessels.
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