Open AccessComparison Between The Efficacy Of Different Laboratory Methods For Diagnosis Of Cryptosporidium
Author(s) -
Mohammed A. Kadir,
Nazakat F. Othman,
Yahya G. Salman
Publication year - 2004
Publication title -
the iraqi journal of veterinary medicine/al-maǧallaẗ al-ṭibbiyyaẗ al-bayṭariyyaẗ al-’irāqiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2410-7409
pISSN - 1609-5693
DOI - 10.30539/ijvm.v28i1.1084
Subject(s) - malachite green , stain , cryptosporidium , methylene blue , staining , safranin , brilliant green , chromatography , microbiology and biotechnology , chemistry , medicine , biology , pathology , feces , organic chemistry , adsorption , photocatalysis , catalysis , biochemistry
This study was performed to detect the effective laboratory method for diagnosis of Cryptosporidium spp. in patients attending the laboratories of Al-Tameem General Hospital and Kirkuk Paediatric Hospital in Kirkuk city, for the period from the beginning of January to end of August 2000..
It was shown that the modified Ziehl-Neelsens stain (hot method) with methylene blue (counter stain) was most efficient in identification of Cryptosporidium oocysts (4.36%), followed by malachite green (counter stain) (1.94%) and modified Ziehl-Neelsen (cold method) with malachite green (counter stain), safranin methylene blue, lugol s iodine preparation (mounting) (0.97%) for each and the modified Ziehl-Neelsen stain (cold method) with methylene blue (counter stain) was the least efficient (0.84%). .