Open AccessRADIOIMMUNOASSAY FOR DETECTION OF ANTI-RINDERPEST ANTIBODIES IN CATTLES
Author(s) -
HM. ALI,
G.S. MOHAMMAD,
HM. ABDULKARIM
Publication year - 2022
Publication title -
the iraqi journal of veterinary medicine/al-maǧallaẗ al-ṭibbiyyaẗ al-bayṭariyyaẗ al-’irāqiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2410-7409
pISSN - 1609-5693
DOI - 10.30539/ijvm.v21i1.1248
Subject(s) - rinderpest , rinderpest virus , antibody , radioimmunoassay , virology , biology , virus , immunology , biochemistry
Liquid Phase Radioimmunoassay (RIA) was developed to detect and measure anti-rinderpest immunoglobulins in field animals sera, within two hours. Rinderpest virus adapted on Vero cell line culture and antigen purified by treatment with Triton-Gentron 13 Butanol, and, labeled with I isotope, using chloramin T iodination method.
Comparative studies for detecting anti-rinderpest immunoglobulin in 80 calves sera samples, using the developed assay in parallel with virus neutralization test (VNT). The study showed 58.75 % agreement between the two methods. However, 71 % of seven months old, non-vaccinated calves showed anti rinderpest antibodies in their sera, also 81 % of 10 months old vaccinated calves were developed antibodies in their blood.
These results demonstrate the development of sensitive, specific and rapid quantitative / qualitative radioimmunoassay, necessary for screening the development of immunity against rinderpest in cattle.