Open AccessEvaluation of the anthelmintic properties of a traditional remedy based on a mixture of red algae using an in vitro assay on gastrointestinal nematodes of donkeys
Author(s) -
Michela Maestrini,
Marcelo Beltrão Molento,
Simone Mancini,
Francesco Saverio Robustelli della Cuna,
Giovanni Furnari,
Donatella Serio,
Laura Cornara,
Stefania Perrucci
Publication year - 2021
Publication title -
open journal of chemistry
Language(s) - English
Resource type - Journals
eISSN - 2618-0758
pISSN - 2618-074X
DOI - 10.30538/psrp-ojc2021.0018
Subject(s) - anthelmintic , serial dilution , biology , algae , laurencia , nematode , red algae , veterinary medicine , haemonchus contortus , zoology , eggs per gram , botany , helminths , ecology , medicine , alternative medicine , pathology
The anthelmintic properties and composition of an Italian traditional anthelmintic remedy based on a red algae mixture (RAE) was assessed using the egg hatch test (EHT). The ability of different dilutions \((1.0, 5.0, 50, or 100%)\) of RAE was determined and compared with the positive and negative controls against gastrointestinal nematode (GIN) of donkeys. The experiment was performed in triplicate. Data were analysed using the ANOVA and Tukey test. In the mixture, Palisada tenerrima, Laurencia intricata and Laurencia spp. red algae were identified. The \(100%\) RAE was able to totally inhibit the egg hatch, showing an efficacy comparable \((P < 0.05)\) to that of the reference drug \((98.7%)\). An egg hatch reduction of \(89.5, 43.7\), and \(23.4%\) was observed at \(50, 5\) and \(1%\) dilutions, respectively. In conclusion, RAE was able to inhibit the egg hatch of GIN of donkeys in a concentration-dependent manner with a correlation coefficient \((R2)\) of \(0.968\), corroborating with its anthelmintic effect.